Global (United States, European Union and China) Sialic Acid Market Research Report 2024-2024
Published date: 2024/09/07
- Location: 13011, Azhikkal, Goa, India
To confirm if both α2,3 and α2,6 sialic acids facilitate AAV1 and AAV6 transduction, we carried out a lectin competition assay on these three cell lines (Fig. 5A to C). In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. If you loved this write-up and you would certainly such as to obtain more info regarding manufacturer of sialic acid powder for pharmaceutical Ingredients kindly see our own web page. The supernatants were removed and used for total sialic acid quantification. Total RNA was extracted using the RNeasy Mini Kit and the RNase-Free DNase Set to eliminate genomic DNA, all from Qiagen (Manchester, UK). Using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA), 1 μg of total RNA was reverse transcribed with random primers.
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