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Published date: 2024/09/07
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US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google PatentsIn competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7. ...
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