US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents
Published date: 2024/09/07
- Location: 90017, Losal, Tamil Nadu, India
In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and finally concentrated to 0.5-1 mm for storage at 4 °C. When you have almost any concerns about in which and how you can work with Supplier of sialic acid powder for cosmetic Ingredients, you possibly can contact us with our own site. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K.
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