US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents
Published date: 2024/09/07
- Location: 4624 Aj, Chinnamanūr, Telangana, India
In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7. Should you have virtually any queries about exactly where and how to utilize Supplier of sialic acid powder as Raw Material for pharmaceuticals, it is possible to email us on the site. 8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and finally concentrated to 0.5-1 mm for storage at 4 °C. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K.
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